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Human Ephrin A5 (EFNA5) ELISA Kit

Catalog Number: orb777963

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb777963
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin A5(EFNA5).
Tested applicationsELISA
ReactivityHuman
Range0.16-10 ng/mL
Assay Time3.5h
Concentration10 ng/mL
UniProt IDP52803
Sensitivity0.053 ng/mL
Alternative namesAF1 ELISA Kit, EFL5 ELISA Kit, EPLG7 ELISA Kit, GL
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NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EFNA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EFNA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA5 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids.
Expiration DatePlease enquire.
Human Ephrin A5 (EFNA5) ELISA Kit