Human Ephrin A1 (EFNA1) ELISA Kit

SKU: orb776855

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin A1(EFNA1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EFNA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EFNA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

B61 ELISA Kit, ECKLG ELISA Kit, EFL1 ELISA Kit, EPLG1 ELISA Kit, LERK1 ELISA Kit, TNFAIP4 ELISA Kit, EPH-related receptor tyrosine kinase ligand 1 ELISA Kit, Tumor necrosis factor alpha-induced protein 4 ELISA Kit, Immediate early response protein B61 ELISA Kit

Quick Database Links

UniProt

P20827