You have no items in your shopping cart.
Cart summary
Human EphA4 Protein, His Tag
Catalog Number: orb612090
| Catalog Number | orb612090 |
|---|---|
| Category | Proteins |
| Description | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EF1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EF5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EF2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. |
| Reactivity | Human |
| Tag | C-10×His |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 60.3 kDa |
| Target | EphA4 |
| UniProt ID | P54764 |
| Protein Sequence | NP_004429.1 |
| Expression System | HEK293 |
| Biological Origin | Human |
| Expression Region | Val 20 - Thr 547 |
| Endotoxins | 1.0 EU per μg |
| NCBI | NP_004429.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Buffer/Preservatives | PBS, pH 7.4 |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Human EPHA4 Protein, His Tag [orb1173840]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 59.2 kDa after removal of the signal peptide. The apparent molecular mass of EPHA4-His is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgEphA4 Protein, Human, Recombinant (aa 570-986, His & GST) [orb1955260]
98.00%
75 kDa (predicted); 67 kDa (reducing conditions)
50 μgEphA4 Protein, Human, Recombinant (His & hFc) [orb1957599]
98.00%
86.5 kDa (predicted); 100-110 kDa (reducing condition, due to glycosylation)
100 μgEphA4 Protein, Human, Recombinant (His) [orb1958156]
98.00%
60 kDa (predicted); 60-70 kDa (reducing condition, due to glycosylation)
1 mg, 100 μgHuman EPHA4 Protein, His & GST Tag [orb2700871]
> 99%, determined by SDS-PAGE
This protein contains the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus and expressed from Baculovirus-Insect Cells.
20 μg, 50 μg
