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| Catalog Number | orb778931 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Endothelin 2(EDN2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Endothelin 2(EDN2). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 15.63-1000 pg/mL |
| Assay Time | 2.5h |
| Concentration | 1000 pg/mL |
| UniProt ID | P20800 |
| Sensitivity | 4.87 pg/mL |
| Alternative names | ET2 ELISA Kit, PPET2 ELISA Kit, Preproendothelin-2 Read more... |
| Note | For research use only |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human EDN2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids. |
| Expiration Date | Please enquire. |
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Human EDN2 ELISA Kit [orb406860]
Human
4.688 pg/mL-300 pg/mL
1.172 pg/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
