Human Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) ELISA Kit

• Catalog Number: orb777697
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electrogenic Sodium Bicarbonate Cotransporter 4(NBC4).
• Reactivity: Human
• Concentration: 20 ng/mL
• UniProt ID: Q9BY07
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NBC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NBC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NBC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NBC4 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.121 ng/mL
• Sample Types: Tissue homogenates and other biological fluids.
• Alternative names: SLC4A5 ELISA Kit, NBCe2 ELISA Kit, Solute Carrier
• Note: For research use only