Human EIF4EBP1(Eukaryotic Translation Initiation Factor 4E Binding Protein 1) ELISA Kit

SKU: orb2999133

Description

Human EIF4EBP1(Eukaryotic Translation Initiation Factor 4E Binding Protein 1) ELISA Kit

Research Area

Signal Transduction

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF4EBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF4EBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF4EBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF4EBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.051 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1; eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1 (PHAS-I);EIF4EBP1

Quick Database Links

UniProt

Q13541