Human EGF R Protein, His Tag

• Catalog Number: orb257415
• Category: Proteins
• Description: Human EGF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 70.5 kDa
• Target: EGF R
• UniProt ID: P00533
• Protein Sequence: NP_005219.2
• Expression System: HEK293
• Biological Origin: Human
• Expression Region: Leu 25 - Ser 645
• Endotoxins: 1.0 EU per μg
• NCBI: NP_005219.2
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Olsen, Dorte Aa et al. Quantification of EGFR autoantibodies in the amplification phenomenon of HER2 in breast cancer Clin Chem Lab Med, 51, 2325-2329 (2013)

PubMed: 24021599

SDS-PAGE analysis of Human EGF R protein

Line graph illustrates about the sensitivity range of Human EGF R protein using different concentrations