Recombinant Human EGF Protein

SKU: orb3002493

Description

Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.

Research Area

Cancer Biology

Key Properties

• Expression System: E.coli
• Biological Activity: Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml (QC verified). Mouse intestinal organoids were cultured with EGF (Cat#orb3002493), Noggin (Cat#orb3002448) and R-spondin 1 (Cat#orb3002497). After five days of culture, the organoids showed good morphology and germination rate. Mouse Colon organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) and R-spondin 1 (Cat#orb3002497). The organoids showed good morphology. Mouse Gastric organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) , R-spondin 1 (Cat#orb3002497) and FGF-10 (Cat#orb3002478). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#orb3002493) , R-spondin 1 (Cat#orb3002497) ,FGF-10 (Cat#orb3002478) and HGF (Cat#orb3002483). The organoids showed good morphology. Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#orb3002480) , BMP-4(Cat#orb3002515) , EGF (Cat#orb3002493) , Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology. Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494) , R-spondin 3 (Cat#orb2993001) , KGF(Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753). The organoids showed good morphology.
• Tag: Tag free
• Expression Region: Asn971-Arg1023
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• MW: Predicted: 6.2 KDa. Observed: 11 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris,200mM NaCl,4%Mannitol,pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone

Quick Database Links

UniProt

P01133