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Catalog Number | orb706925 |
---|---|
Category | Proteins |
Description | Recombinant Human ECE1 Protein is produced by mammalian expression system and the target gene encoding Gln90-Trp770 is expressed with a 8His tag at the N-terminus. |
Target | ECE1 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Protein Sequence | HHHHHHHHQY QTRSPSVCLS EACVSVTSSI LSSMDPTVDP CHDFFSYACG GWIKANPVPD GHSRWGTFSN LWEHNQAIIK HLLENSTASV SEAERKAQVY YRACMNETRI EELRAKPLME LIERLGGWNI TGPWAKDNFQ DTLQVVTAHY RTSPFFSVYV SADSKNSNSN VIQVDQSGLG LPSRDYYLNK TENEKVLTGY LNYMVQLGKL LGGGDEEAIR PQMQQILDFE TALANITIPQ EKRRDEELIY HKVTAAELQT LAPAINWLPF LNTIFYPVEI NESEPIVVYD KEYLEQISTL INTTDRCLLN NYMIWNLVRK TSSFLDQRFQ DADEKFMEVM YGTKKTCLPR WKFCVSDTEN NLGFALGPMF VKATFAEDSK SIATEIILEI KKAFEESLST LKWMDEETRK SAKEKADAIY NMIGYPNFIM DPKELDKVFN DYTAVPDLYF ENAMRFFNFS WRVTADQLRK APNRDQWSMT PPMVNAYYSP TKNEIVFPAG ILQAPFYTRS SPKALNFGGI GVVVGHELTH AFDDQGREYD KDGNLRPWWK NSSVEAFKRQ TECMVEQYSN YSVNGEPVNG RHTLGENIAD NGGLKAAYRA YQNWVKKNGA EHSLPTLGLT NNQLFFLGFA QVWCSVRTPE SSHEGLITDP HSPSRFRVIG SLSNSKEFSE HFRCPPGSPM NPPHKCEVW |
UniProt ID | P42892 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | Endothelin-converting enzyme 1 protein, ECE-1 prot Read more... |
Note | For research use only |
Entrez | 1889 |
WB | |
Human | |
Mouse | |
Polyclonal | |
Unconjugated |
98.00% | |
80 kDa (predicted); 125 kDa (reducing condition, due to glycosylation) |
> 95%, determined by SDS-PAGE | |
This protein contains human ECE1 isoform B (P42892-1) (Gln 90-Trp 770) was expressed with a polyhistidine tag at the N-terminus and expressed from HEK293 Cells. |