Human E3 Ubiquitin-Protein Ligase CHIP (STUB1) ELISA Kit

SKU: orb1146733

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E3 ubiquitin-protein ligase CHIP(STUB1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E3 ubiquitin-protein ligase CHIP(STUB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E3 ubiquitin-protein ligase CHIP(STUB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E3 ubiquitin-protein ligase CHIP(STUB1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STUB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STUB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STUB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STUB1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 18.74 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

STUB1, CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1, STIP1 homology and U-box containing protein 1

Quick Database Links

UniProt

Q9UNE7