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Human Dynamin 1 (DNM1) ELISA Kit

Catalog Number: orb776574

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776574
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynamin 1(DNM1).
ReactivityHuman
Concentration20 ng/mL
UniProt IDQ05193
Tested applicationsELISA
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNM1 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.111 ng/mL
Sample Typestissue homogenates, cell lysates and other biological fluids
Alternative namesB dynamin , D100 , DNM 1 , DNM , DNM1 , Dynamin ,
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NoteFor research use only
Human Dynamin 1 (DNM1) ELISA Kit

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