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Human Dopamine (DA) ELISA Kit

Catalog Number: orb1291358

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb1291358
CategoryAssays and Kits
DescriptionHuman DA (Dopamine) ELISA Kit
ReactivityHuman
Concentration1000 pg/mL
Tested applicationsELISA
Application notesstandard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human DA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DA in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range15.63-1000 pg/mL
Sensitivity4.53 pg/mL
Sample Typesserum, plasma and other biological fluids
Alternative names2-(3,4-Dihydroxyphenyl)ethylamine, 3,4-Dihydroxyph
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NoteFor research use only
Human Dopamine (DA) ELISA Kit

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