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Catalog Number | orb1291358 |
---|---|
Category | Assays and Kits |
Description | Human DA (Dopamine) ELISA Kit |
Reactivity | Human |
Concentration | 1000 pg/mL |
Tested applications | ELISA |
Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human DA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DA in the samples is then determined by comparing the OD of the samples to the standard curve |
Assay Type | Competitive |
Assay Time | 2.5h |
Range | 15.63-1000 pg/mL |
Sensitivity | 4.53 pg/mL |
Sample Types | serum, plasma and other biological fluids |
Alternative names | 2-(3,4-Dihydroxyphenyl)ethylamine, 3,4-Dihydroxyph Read more... |
Note | For research use only |
Human | |
0.5 ng/mL-100 ng/mL | |
0.25 ng/mL |