Human Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) ELISA Kit

SKU: orb778244

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1(DPAGT1).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPAGT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DPAGT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DPAGT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DPAGT1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.061 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ALG7 ELISA Kit, CDG-Ij ELISA Kit, DGPT ELISA Kit, DPAGT ELISA Kit, DPAGT2 ELISA Kit, G1PT ELISA Kit, GPT ELISA Kit, UAGT ELISA Kit, UGAT ELISA Kit, GlcNAc-1-P Transferase ELISA Kit, UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA Kit

Quick Database Links

UniProt

Q9H3H5