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Item 1 of 4
Item 1 of 4
Human DNAM-1 Protein, mFc-His Tag
Catalog Number: orb689406
| Catalog Number | orb689406 |
|---|---|
| Category | Proteins |
| Description | Recombinant human DNAM-1 protein with C-terminal mouse Fc and 6×His tag |
| Reactivity | Human |
| Tag | C-Mouse Fc and 6×His Tag |
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| MW | The protein has a predicted molecular mass of 53.5 kDa after removal of the signal peptide. |
| Target | DNAM1 |
| UniProt ID | Q15762 |
| Source | Mammalian |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Alternative names | DNAM1;CD226;PTA1 Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Human PD-1 Protein, hFc-His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human PD1, hFc-His tagged protein (orb689448) can bind Human PDL1, mFc-His tagged protein orb689384 in a linear range of 62.5-251.1 ng/ml.
Flow cytometry analysis with 0.2 μg/ml Human PD-1 Protein, hFc-His tag (orb689448) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2 μg/mL Human PD-1 Protein, hFc-His tag (orb689448) followed by PE-conjugated Goat anti-human IgG secondary antibody.
