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Catalog Number | orb1291804 |
---|---|
Category | Proteins |
Description | Human DLK1, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Gly 303 (Accession # P80370-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 31.7 kDa |
Target | DLK1 |
UniProt ID | P80370 |
Protein Sequence | Ala 24 - Gly 303 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Ala 24 - Gly 303 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 30.6 kDa after removal of the signal peptide. The apparent molecular mass of DLK1-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
30.9 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |