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Human DKK1 Protein

Catalog Number: orb1476706

Select Product Size
SizePriceQuantity
20 μg$ 150.00
100 μg$ 260.00
1 mg$ 1,200.00
20 μg Enquire
100 μg Enquire
1 mg Enquire
DispatchUsually dispatched within 5-10 working days
Catalog Numberorb1476706
CategoryProteins
DescriptionRecombinant Human Dickkopf-related protein 1(DKK1) (Active)
TagC-terminal 10xHis-tagged
Form/AppearanceLyophilized powder
Buffer/PreservativesLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
PurityGreater than 95% as determined by SDS-PAGE.
Protein SequenceTLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH
Protein LengthFull Length of Mature Protein
UniProt IDO94907
MW28.5 kDa
Application notesWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
EndotoxinsLess than 1.0 EU/ug as determined by LAL method.
SourceMammalian cell
Biological OriginHomo sapiens (Human)
Biological ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human DKK1 at 2 μg/mL can bind Anti-DKK1 recombinant antibody, the EC50 is 1.283-2.544 ng/mL.
Expression Region32-266aa
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Alternative names(Dickkopf-1)(Dkk-1)( hDkk-1)(SK)
Research AreaCardiovascular Research
NoteFor research use only
Expiration Date6 months from date of receipt.
Human DKK1 Protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Human DKK1 Protein

Measured by its binding ability in a functional ELISA. Immobilized Human DKK1 at 2 μg/mL can bind Anti-DKK1 recombinant antibody, the EC50 is 1.283-2.544 ng/mL.

  • Human DKK1 Protein, hFc Tag [orb1173930]

    The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 51.9 kDa after removal of the signal peptide.The apparent molecular mass of DKK1-hFC is approximately 70 kDa due to glycosylation.

    Mammalian

    50 μg, 10 μg, 100 μg
  • Human DKK1 protein [orb418774]

    Greater than 90% as determined by SDS-PAGE.

    29.7 kDa

    E.coli

    20 μg, 100 μg, 1 mg
  • Human DKK1 ELISA Kit [orb390891]

    Human

    62.5 pg/ml - 4000 pg/ml

    10 pg/ml

    96 T
  • Human DKK1 Protein, mFc Tag [orb1743312]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 52.0 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-mFc is approximately 55-70 kDa due to glycosylation.

    E.coli

    10 μg, 50 μg, 100 μg
  • Human DKK1 Protein, His Tag [orb1743317]

    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 26.6 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-His is approximately 35-55 kDa due to glycosylation.

    E.coli

    10 μg, 50 μg, 100 μg