Human Discoidin Domain Receptor Family, Member 2 (DDR2) ELISA Kit

SKU: orb778862

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discoidin Domain Receptor Family, Member 2(DDR2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DDR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DDR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DDR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DDR2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.52 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD167b ELISA Kit, MIG20a ELISA Kit, NTRKR3 ELISA Kit, TKT ELISA Kit, TYRO10 ELISA Kit, Discoidin Domain Receptor Tyrosine Kinase 2 ELISA Kit, Neurotrophic tyrosine kinase, receptor-related 3 ELISA Kit, Receptor protein-tyrosine kinase TKT ELISA Kit

Quick Database Links

UniProt

Q16832