Human Dipeptidyl Peptidase 10 (DPP10) ELISA Kit

SKU: orb776931

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 10(DPP10).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DPP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DPP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DPP10 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.117 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

DPL2 ELISA Kit, DPPY ELISA Kit, DPRP3 ELISA Kit, Dipeptidylpeptidase 10(Non-Functional) ELISA Kit, Dipeptidyl peptidase IV-related protein 3 ELISA Kit, Dipeptidyl peptidase X ELISA Kit, Dipeptidyl peptidase-like protein 2 ELISA Kit

Quick Database Links

UniProt

Q8N608