Human Dihydrotestosterone (DHT) ELISA Kit

SKU: orb782539

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dihydrotestosterone(DHT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dihydrotestosterone(DHT).

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human DHT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DHT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DHT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 39.07-2500 pg/mL
• Sensitivity: 13.2 pg/mL
• Concentration: 2500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

5α-DHT ELISA Kit, 5α-Dihydrotestosterone ELISA Kit, Androstanolone ELISA Kit, Stanolone ELISA Kit