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| Catalog Number | orb2667984 |
|---|---|
| Category | Assays and Kits |
| Description | Human Deoxyribonuclease I (DNASE1) ELISA Kit |
| UniProt ID | P24855 |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNASE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNASE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNASE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNASE1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.098 ng/mL |
| Sample Types | Serum, plasma, urine, saliva, seminal plasma and other biological fluids |
| Alternative names | DNaseI; DNL1; DRNI; DNase-I; Dornase alfa |
| Note | For research use only |
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Human DNASE1 ELISA Kit [orb407195]
Human
1.56 ng/mL-100 ng/mL
0.39 ng/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
