Human Delta-Sleep Inducing Peptide (dSIP) ELISA Kit

SKU: orb775810

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Delta-Sleep Inducing Peptide(dSIP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Delta-Sleep Inducing Peptide(dSIP).

Research Area

Metabolism Research, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human dSIP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dSIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dSIP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 23.44-1500 pg/mL
• Sensitivity: 7.49 pg/mL
• Concentration: 1500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

DSIPI ELISA Kit, TSC22D3 ELISA Kit, DIP ELISA Kit, GILZ ELISA Kit, TSC-22R ELISA Kit, hDIP ELISA Kit, TSC22 Domain Family Protein 3 ELISA Kit, Glucocorticoid-Induced Leucine Zipper ELISA Kit, Delta Sleep Inducing Peptide,Immunoreactor ELISA Kit