Human Delta-Sleep Inducing Peptide (dSIP) ELISA Kit

• Catalog Number: orb775810
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Delta-Sleep Inducing Peptide(dSIP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Delta-Sleep Inducing Peptide(dSIP).
• Reactivity: Human
• Concentration: 1500 pg/mL
• Tested applications: ELISA
• Application notes: standard: 1500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human dSIP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dSIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dSIP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 23.44-1500 pg/mL
• Sensitivity: 7.49 pg/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Alternative names: DSIPI ELISA Kit, TSC22D3 ELISA Kit, DIP ELISA Kit,
• Note: For research use only