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Catalog Number | orb391616 |
---|---|
Category | Proteins |
Description | Recombinant human DEFB4A protein is produced by our E. coli expression system and the target gene encoding Gly24-Pro64 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 130mM NaCl, pH 7.4 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | DEFB4A; DEFB4B |
Entrez | 1673, 100289462 |
UniProt ID | O15263 |
Protein Sequence | GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 130mM NaCl, pH 7.4 |
Alternative names | DEFB102; DEFB2; DEFB4; Beta-defensin 4A; Beta-defe Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
31.3 kDa | |
E.coli |
> 90% as determined by SDS-PAGE | |
22.3 kDa |
98.00% | |
9 KDa (reducing condition) |