Human DEAD Box Polypeptide 5 (DDX5) ELISA Kit

SKU: orb779994

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEAD Box Polypeptide 5(DDX5).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DDX5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DDX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DDX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DDX5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.062 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

G17P1 ELISA Kit, HLR1 ELISA Kit, HELR ELISA Kit, HUMP68 ELISA Kit, p68 ELISA Kit, Asp-Glu-Ala-Asp Box Polypeptide 5 ELISA Kit, Probable ATP-dependent RNA helicase DDX5 ELISA Kit

Quick Database Links

UniProt

P17844