Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

• Catalog Number: orb775679
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4(CTLA4).
• Reactivity: Human
• Concentration: 2000 pg/mL
• UniProt ID: P16410
• Tested applications: ELISA
• Application notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CTLA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTLA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CTLA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTLA4 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.9 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Alternative names: CD152 ELISA Kit, CELIAC3 ELISA Kit, CTLA4 ELISA Ki
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only