Human Cytokeratin 18 (CK-18) ELISA Kit

SKU: orb782370

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CK-18(Cytokeratin 18).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK-18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK-18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK-18 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Cell proliferation inducing gene 46 protein ELISA Kit, Cell proliferation inducing protein 46 ELISA Kit, Cell proliferation-inducing gene 46 protein ELISA Kit, CK 18 ELISA Kit, CK-18 ELISA Kit, CK18 ELISA Kit, CYK 18 ELISA Kit, CYK18 ELISA Kit, Cytokeratin 18 ELISA Kit, Cytokeratin endo B ELISA Kit, Cytokeratin-18 ELISA Kit, K 18 ELISA Kit, K18 ELISA Kit, K1C18_HUMAN ELISA Kit, KA18 ELISA Kit, Keratin 18 ELISA Kit, Keratin 18, type I ELISA Kit, Keratin D ELISA Kit, keratin, type I cytoskeletal 18 ELISA Kit, Keratin-18 ELISA Kit, Krt18 ELISA Kit