Human Cytochrome P450 2E1 (CYP2E1) ELISA Kit

SKU: orb778327

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1(CYP2E1).

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP2E1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP2E1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP2E1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.32 ng/mL
• Concentration: 50 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CPE1 ELISA Kit, CYPIIE1 ELISA Kit, P450-J ELISA Kit, P450C2E ELISA Kit, 4-nitrophenol 2-hydroxylase ELISA Kit, Cytochrome P450 Family 2 Subfamily E 1 ELISA Kit, Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1 ELISA Kit

Quick Database Links

UniProt

P05181