Human Cysteine Rich Secretory Protein 3 (CRISP3) ELISA Kit

SKU: orb777844

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Secretory Protein 3(CRISP3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 60 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRISP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRISP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CRISP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRISP3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, saliva, sweat and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.94-60 ng/mL
• Sensitivity: 0.45 ng/mL
• Concentration: 60 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CRS3 ELISA Kit, Aeg2 ELISA Kit, CRISP-3 ELISA Kit, SGP28 ELISA Kit, Specific granule protein of 28 kDa ELISA Kit

Quick Database Links

UniProt

P54108