Human Cystatin-F (CST7) ELISA Kit

SKU: orb2668221

Description

Human Cystatin-F (CST7) ELISA Kit

Research Area

Cell Biology

Images & Validation

• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CST7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CST7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CST7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CST7 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma,cell culture supernates
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.625-40 ng/mL
• Sensitivity: 0.156 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CST7;Cystatin-F;Cystatin-7;Cystatin-like metastasis-associated protein ;CMAP;Leukocystatin

Quick Database Links

UniProt

O76096