Human CYP27A1(Cytochrome P450 27A1) Microsample ELISA Kit

• Catalog Number: orb2998254
• Category: Assays and Kits
• Description: Human CYP27A1(Cytochrome P450 27A1) Microsample ELISA Kit
• UniProt ID: Q02318
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP27A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP27A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP27A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP27A1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Alternative names: CP27; CTX; CYP27; Sterol 27-Hydroxylase,Cerebroten
• Note: For research use only
• Expiration Date: Please enquire.