Human Cylindromatosis (CYLD) ELISA Kit

SKU: orb778392

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cylindromatosis(CYLD).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYLD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYLD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYLD in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.115 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CDMT ELISA Kit, CYLD1 ELISA Kit, CYLDI ELISA Kit, EAC ELISA Kit, USPL2 ELISA Kit, Turban Tumor Syndrome ELISA Kit, Ubiquitin Carboxyl-Terminal Hydrolase CYLD ELISA Kit, Ubiquitin Specific Peptidase Like 2 ELISA Kit, Deubiquitinating enzyme CYLD ELISA Kit

Quick Database Links

UniProt

Q9NQC7