Human Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ELISA Kit

SKU: orb775324

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 2A(CDKN2A).

Research Area

Cancer Biology, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDKN2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDKN2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDKN2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDKN2A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.267 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

P16 ELISA Kit, ARF ELISA Kit, CDK4I ELISA Kit, CMM2 ELISA Kit, INK4 ELISA Kit, INK4a ELISA Kit, MLM ELISA Kit, MTS1 ELISA Kit, TP16 ELISA Kit, P14 ELISA Kit, P14ARF ELISA Kit, P16INK4a ELISA Kit, P19 ELISA Kit, Melanoma P16 ELISA Kit, Cyclin-dependent kinase 4 inhibitor A ELISA Kit, Multiple tumor suppressor 1 ELISA Kit

Quick Database Links

UniProt

P42771