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Catalog Number | orb545757 |
---|---|
Category | Proteins |
Description | Human CTGF, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 37.4 kDa |
Target | CTGF, CCN2 |
UniProt ID | Q5M8T4 |
Source | Human CTGF, His Tag (orb545757) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Human CTGF, His Tag with an affinity constant of 6.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Gln 27 - Ala 349 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001892.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Potassium glutamate and Arginine, pH7.0 |
Alternative names | CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding p Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.4 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Human CTGF, His Tag (Cat. No. orb545757) with an affinity constant of 6.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Unconjugated | |
90% | |
37.3 kDa | |
Rhesus macaque CTGF, His Tag (orb668877) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # H9FQD5-1). |
Unconjugated | |
90% | |
37.3 kDa | |
Mouse CTGF, His Tag (orb545763) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Ala 348 (Accession # P29268-1). |
98.00% | |
15.1 kDa (predicted) |
Greater than 95.0% as determined by SDS-PAGE. | |
HEK293 cells |
> 95% as determined by Bis-Tris PAGE | |
38.4 kDa (predicted). Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result. |