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Catalog Number | orb867319 |
---|---|
Category | Proteins |
Description | Human CTGF, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln27-Ala349 (Accession # Q5M8T4-1 ). |
Reactivity | Human |
Tag | N-Fc |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 61.9 kDa |
Target | CTGF, CCN2 |
UniProt ID | Q5M8T4 |
Source | Human CTGF, Fc Tag (orb867319) is expressed from human 293 cells (HEK293). It contains AA Gln27-Ala349 (Accession # Q5M8T4-1 ). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human CTGF, Fc Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.8-6 ng/mL (QC tested). |
Expression Region | Gln27-Ala349 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001892.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 |
Alternative names | CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding p Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 61.9 kDa. The protein migrates as 48-53 kDa and 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human CTGF, Fc Tag (Cat. No. orb867319) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.8-6 ng/mL (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 61.6 kDa after removal of the signal peptide. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
98.00% | |
67-81 KDa (reducing condition) |