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| Catalog Number | orb757438 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human CSPG4 Protein with C-terminal 6×His tag. |
| Target | CSPG4 |
| Tag | C-6×His Tag |
| Reactivity | Human |
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| UniProt ID | Q6UVK1 |
| MW | The protein has a predicted molecular mass of 237.7 kDa after removal of the signal peptide. |
| Source | Mammalian |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Alternative names | CSPG4A;HMW-MAA;MCSP;MCSPG;MEL-CSPG;MSK16;NG2 |
| Research Area | Cancer, Developmental Biology, Epigenetics |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

Human TIGIT Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD155, mFc-His tagged protein ([getskuurl sku"orb689388"]) can bind Human TIGIT, hFc tagged protein (orb689442) in a linear range of 1.95-125 ng/ml.

Flow cytometry analysis with 1 μg/mL Human TIGIT Protein, hFc tag (orb689442) on Expi293 cells transfected with human CD112 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TIGIT Protein, hFc Tag (orb689442) can bind Anti-TIGIT (etigilimab biosimilar) mAb (orb689329) in a linear range of 0.32–8 ng/ml.
>90% as determined by SDS-PAGE. | |
27.34 kDa |
>90% as determined by SDS-PAGE | |
31.4 kDa |
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