Human Cross Linked N-Telopeptide of Type I Collagen (NTXI) ELISA Kit

SKU: orb775707

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cross Linked N-Telopeptide Of Type I Collagen(NTXI) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cross Linked N-Telopeptide Of Type I Collagen(NTXI).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NTXI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTXI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 0.94 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NTX-I ELISA Kit, NTX1 ELISA Kit