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Catalog Number | orb775963 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cross Linked C-Telopeptide Of Type I Collagen(CTXI) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cross Linked C-Telopeptide Of Type I Collagen(CTXI). |
Tested applications | ELISA |
Reactivity | Human |
Range | 156.25-10000 pg/mL |
Assay Time | 2.5h |
Concentration | 10000 pg/mL |
Sensitivity | 50.5 pg/mL |
Alternative names | CTX-I ELISA Kit, CTX1 ELISA Kit, ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CTXI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTXI in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
Human | |
0.156-10ng/ml | |
0.094ng/ml |