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Human Complement Factor I (CFI) ELISA Kit

Catalog Number: orb1947737

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1947737
CategoryAssays and Kits
DescriptionHuman Complement Factor I (CFI) ELISA Kit
TargetCFI
ReactivityHuman
UniProt IDP05156
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFI. You can calculate the concentration of Human CFI in the samples by comparing the OD of the samples to the standard curve.
Assay Time3.5H
Range3.13-200ng/mL
Sensitivity1.88ng/mL
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Alternative namesCFⅠ
NoteFor research use only
Human Complement Factor I (CFI) ELISA Kit

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    0.051ng/mL

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