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Catalog Number | orb612152 |
---|---|
Category | Proteins |
Description | Human Complement Factor D, His Tag is expressed from human 293 cells (HEK293) and active. It contains AA Ile 26 - Ala 253 (Accession # P00746-1). |
Target | Complement Factor D |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 8.0 |
Purity | 95% |
Protein Sequence | NP_001919.2 |
UniProt ID | P00746 |
MW | 26.3 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.3 kDa. The protein migrates as 28 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Ile 26 - Ala 253 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001919.2 |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
26.5 kDa |
98.00% | |
25.8 kDa (predicted); 33 kDa (reducing condition, due to glycosylation) |
> 95% by SDS-PAGE. | |
Recombinant Human Complement Factor D/Adipsin Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Ile26-Ala253) of human Complement Factor D/Adipsin (Accession #P00746) fused with a 10��His Tag at the C-terminus. |
> 97%, determined by SDS-PAGE | |
This protein contains the mature form of human CFD (P00746) (Ile 26-Ala 253) was fused with a C-terminal polyhistidine tag and a signal peptide at the N-terminus and expressed from HEK293 Cells. |