Human Complement Factor D (CFD) ELISA Kit

SKU: orb774993

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D(CFD).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFD in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.254 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CF-D ELISA Kit, DF ELISA Kit, PFD ELISA Kit, ADN ELISA Kit, Adipsin ELISA Kit, Properdin Factor D ELISA Kit, C3 convertase activator ELISA Kit

Standard curve for orb774993 ELISA kit.

Quick Database Links

UniProt

P00746