You have no items in your shopping cart.
Cart summary
| Catalog Number | orb780274 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4d(C4d). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 2.19-140 ng/mL |
| Assay Time | 3.5h |
| Concentration | 140 ng/mL |
| Sensitivity | 0.96 ng/mL |
| Alternative names | C4d Read more... |
| Note | For research use only |
| Application notes | standard: 140 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4d in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
