Human Complement Component 1, R Subcomponent (C1r) ELISA Kit

SKU: orb2667781

Description

Human Complement Component 1, R Subcomponent (C1r) ELISA Kit

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Application Notes:
  standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1r. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1r. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1r, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1r in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.29 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C1-r; complement Component 1; Complement component 1 subcomponent r

Quick Database Links

UniProt

P00736