Human Complement C5a Protein, Fc Tag

• Catalog Number: orb612128
• Category: Proteins
• Description: Human Complement C5a, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Leu 679 - Arg 751 (Accession # P01031-1).
• Target: Complement C5a
• Conjugation: Unconjugated
• Tag: C-Fc
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH 7.5
• Purity: 90%
• UniProt ID: P01031
• MW: 34.6 kDa
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Leu 679 - Arg 751
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Immobilized Human Complement C5a, Fc Tag (Cat. No. orb612128) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 2-16 ng/mL (QC tested).