Human Complement C5a Protein

• Catalog Number: orb1496182
• Category: Proteins
• Description: Complement Component C5a (C5a) is also known as C5,and is a protein fragment released from complement component C5.,is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.
• Reactivity: Human
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 10.2 kDa
• Target: Complement C5a
• Source: Human Complement C5a, His Tag (orb1496182) is expressed from E. coli cells. It contains AA Leu 679 - Arg 751 (Accession # P01031-1).
• Expression System: E. coli
• Biological Activity: Immobilized Human Complement C5a, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-4 ng/ml (QC tested).
• Expression Region: Leu 679 - Arg 751
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: C5a,Complement Component 5a
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 10.2 kDa. The protein migrates as 14-15 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

Immobilized Human Complement C5a, His Tag (Cat. No. orb1496182) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-4 ng/ml (QC tested).