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Human Complement C5 Protein, His-Avi Tag (Biotin)
Catalog Number: orb1496162
| Catalog Number | orb1496162 |
|---|---|
| Category | Proteins |
| Description | Biotinylated Human Complement C5, His, is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1). |
| Reactivity | Human |
| Tag | C-10×His & C-Avi |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Biotin |
| MW | 189.9 kDa |
| Target | Complement C5 |
| UniProt ID | P01031 |
| Protein Sequence | NP_001726.2 |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Immobilized Biotinylated Human Complement C5, His, (Cat. No. ) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested). |
| Expression Region | Gln 19 - Cys 1676 |
| Endotoxins | 1.0 EU per μg |
| NCBI | NP_001726.2 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Buffer/Preservatives | PBS, pH 7.4 |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag . The protein has a calculated MW of 189.9 kDa. As a result of glycosylation, the protein migrates as 75 kDa, 120 kDa and 200 kDa under reducing (R) condition, and 200 kDa under non-reducing (NR) condition (SDS-PAGE). |
| Expiration Date | 6 months from date of receipt. |
Immobilized Biotinylated Human Complement C5, His, Avitag (Cat. No. orb1496162) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
