Human Complement 1 Inhibitor (C1INH) ELISA Kit

SKU: orb775787

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor(C1INH).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1INH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1INH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1INH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1INH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.42 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SERPING1 ELISA Kit, C1IN ELISA Kit, C1-INH ELISA Kit, C1NH ELISA Kit, HAE1 ELISA Kit, HAE2 ELISA Kit, Serpin Peptidase Inhibitor Clade G Member 1 ELISA Kit, Angioedema Hereditary ELISA Kit, C1 Inhibitor ELISA Kit, C1 Esterase Inhibitor ELISA Kit

Quick Database Links

UniProt

P05155