Human Collagen Type IV protein

• Catalog Number: orb750384
• Category: Proteins
• Description: Human Collagen Type IV protein
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Concentration: 1.0 mg/ml
• Dilution range: IF: 1:100-1:500, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with Rockland’s anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).
• Conjugation: Unconjugated
• Target: COL4A1-COL4A6
• UniProt ID: P02462
• Source: Human
• Biological Origin: Human
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Type IV collagen protein, collagen 4 protein, huma
• Note: For research use only
• Application notes: For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.
• Expiration Date: 6 months from date of receipt.

Knockdown of NORAD inhibits proliferation, inflammation and fibrosis in HG-induced HMCs. Levels of (G) Col. IV in HMCs measured via ELISA. **P < 0.01 vs. NG; ##P < 0.01 vs. HG + si-NC. NORAD, non-coding RNA activated by DNA damage; NG, normal glucose; HG, high glucose; HMCs, human mesangial cells; si, small interfering RNA; NC, negative control Col. IV, type IV collagen.

NORAD knockdown inhibits proliferation, inflammation and fibrosis in HG-induced HMCs by regulating the miR-485/NRF1 axis. (G) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs as determined via ELISA. **P < 0.01 vs. si-NC; #P < 0.05 vs. si-NORAD + inhibitor NC; &P < 0.05 vs. si-NORAD + ov-NC. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; NRF1, nuclear respiratory factor 1; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; si, small interfering RNA; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1.

NORAD reverses the inhibitory effects of miR-485 on HG-induced HMCs. (F) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs measured via ELISA **P < 0.01 vs. miR-NC; #P < 0.05 vs. miR-485 mimics. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1.

SDS-PAGE Results of Human Collagen Type IV. Lane 1: Human Collagen Type IV Reduced (5.0 µg). Lane 2: Opal Prestained Molecular Weight Protein. Lane 3: Human Collagen Type IV Non-Reduced (5.0 µg). 4-20% Gel, Coomassie Stained. Observed MW: Type IV α1, α2, α3 bands.