Human Collagen Type III protein

• Catalog Number: orb750383
• Category: Proteins
• Description: Human Collagen Type III protein
• Target: COL3A1
• Conjugation: Unconjugated
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with Anti-Collagen Type III. Reaction with Rockland’s Anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
• UniProt ID: P02461
• Tested applications: ELISA, IHC, IP, SDS-PAGE, WB
• Dilution range: IF: 1:100-1:500, IP: 1:100-1:500, WB: 1:1000
• Application notes: Human Collagen Type III purified protein standard is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
• Source: Human
• Biological Origin: Human
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Type III collagen protein, collagen 3 protein, hum
• Note: For research use only
• NCBI: NP_000081.1

SDS-PAGE Results of Human Collagen Type III. Lane 1: Human Collagen Type III Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human Collagen Type III Non-Reduced [1.0 µg]. 4-20% Gel Coomassie Stained. Observed: α1 bands.

Western Blot of COLLAGEN III. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C. Secondary antibody: DyLight™ 649 Goat anti-rabbit at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.