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Catalog Number | orb2667698 |
---|---|
Category | Assays and Kits |
Description | Human Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit |
Range | 3.13-200 ng/mL |
Assay Time | 2.5h |
UniProt ID | P05160 |
Sensitivity | 1.24 ng/mL |
Alternative names | F13-B; FXIIIB; Fibrin-stabilizing factor B subunit Read more... |
Note | For research use only |
Application notes | standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | plasma |
Expiration Date | Please enquire. |