Human Cluster ofDifferentiation 8 (CD8) ELISA Kit

SKU: orb778966

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 8a(CD8a).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD8 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.62 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

p32 ELISA Kit, CD8-A ELISA Kit, Leu2 ELISA Kit, MAL ELISA Kit, T-cell surface glycoprotein CD8 alpha chain ELISA Kit, T-lymphocyte differentiation antigen T8/Leu-2 ELISA Kit

Quick Database Links

UniProt

P01732