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Human CLDN18.2 full length protein-synthetic nanodisc

Catalog Number: orb1291760

DispatchUsually dispatched within 5-10 working days
$ 940.00
Catalog Numberorb1291760
CategoryProteins
DescriptionHuman CLDN18.2 full length protein-synthetic nanodisc
TargetCLDN18.2
TagC-Flag Tag
ReactivityHuman
Form/AppearanceLyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments.
UniProt IDP56856
MWThe human full length CLDN18.2 Protein has a MW of 27.5 kDa
Expression SystemHEK293
SourceMammalian
StorageStore at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Alternative namesCLDN18.2
NoteFor research use only
Human CLDN18.2 full length protein-synthetic nanodisc

Elisa plates were added with Flag Tag CLDN18.2-Nanodisc (0.2 μg/per well) on an anti-Flag monoclonal antibody pre-coated (0.2 μg/per well) plate. Serial diluted anti- CLDN18.2 monoclonal antibody (orb1173630) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-Nanodisc is 593.6 ng/ml.

Human CLDN18.2 full length protein-synthetic nanodisc

Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGE

Human CLDN18.2 full length protein-synthetic nanodisc

Human CLDN18.2-Nanodisc can bind Anti-CLDN18.2 antibody (orb1173630) with an affinity constant of 1.619 nM as determined in a SPR assay.