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Catalog Number | orb1291760 |
---|---|
Category | Proteins |
Description | Human CLDN18.2 full length protein-synthetic nanodisc |
Target | CLDN18.2 |
Tag | C-Flag Tag |
Reactivity | Human |
Form/Appearance | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
UniProt ID | P56856 |
MW | The human full length CLDN18.2 Protein has a MW of 27.5 kDa |
Expression System | HEK293 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | CLDN18.2 |
Note | For research use only |
Elisa plates were added with Flag Tag CLDN18.2-Nanodisc (0.2 μg/per well) on an anti-Flag monoclonal antibody pre-coated (0.2 μg/per well) plate. Serial diluted anti- CLDN18.2 monoclonal antibody (orb1173630) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-Nanodisc is 593.6 ng/ml.
Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGE
Human CLDN18.2-Nanodisc can bind Anti-CLDN18.2 antibody (orb1173630) with an affinity constant of 1.619 nM as determined in a SPR assay.
The human full length CLDN18.2-Strep Protein has a MW of 27.5 kDa |
The human full length CLDN18.2 Protein has a MW of 32.5 kDa |